In conclusion, as shown in Fig. 7, this study first provides and verifies the solid evidences that EA treatment enhances neurobehavioral functional recovery through miR-181b targeting PirB after ischemic stroke, suggesting that miR-181b and PirB act as key regulators of axon regeneration and CST projection after cerebral ischemic-reperfusion injury. The gene discussed is LILRB3; the disease is ischemic stroke.