Therefore, we speculated that miRNA-186-mediated crosstalk between PVT1 and PTTG1 existed in adjacent tissues and maintained normal functions, and the “loss” of PVT1-PTTG1 crosstalk might act as a driver event implicated in development of the subtype 1 of ESCC. The gene discussed is PTTG1; the disease is esophageal squamous cell carcinoma.