The indicated levels are as follows: (A) 3.9 nmol/g, threshold for mortality in the offspring (see Supplementary Results); (B) 8.3 nmol/g, threshold for 20% reduced growth24; (C) 12 nmol/g, threshold for liver TK latency (Fig. 5e); and (D) 17–19 nmol/g, suggested threshold range for sublethal thiamine deficiency (see Supplementary Results). This evidence concerns the gene TKT and Thiamine deficiency.