In addition, the protein expression of DUSP4 was detected in different GC cell lines (AGS, MGC‐803, SGC‐7901, HGC‐27 and BGC‐823) by Western blotting, indicating that DUSP4 expression level was markedly down‐regulated in SGC‐7901 and HGC‐27 cell lines compared with other ones (Fig. 1B). This evidence concerns the gene DUSP4 and gastric cancer.