We demonstrated for the first time that intragastric administration of 3DG to rats for 2 weeks led to an obvious increase in 3DG content of the upper small intestine, lower small intestine, ileum and colon, and reduced plasma total GLP-1 and insulin concentrations in a similar manner, in conjunction with increased fasting blood glucose concentration and reduced oral glucose tolerance. This evidence concerns the gene GLP1R and glucose measurement.