CP and hereditary spastic paraplegia: After 8 h treatment with TTX or TTX/Aβ, both conditions showed a ~10% increase in mEPSC amplitude over controls, and addition of PhTx during recordings reduced amplitudes back to control levels (Fig. 3a2), indicating that the small increase in mEPSC amplitude during HSP initiation predominantly resulted from addition of CP-AMPARs in both TTX and TTX/Aβ treated neurons.