SLC20A2 and primary biliary cholangitis: To simulate the effect of a monoallelic variant in the SLC20A2 gene and thus mimic the PBC disease situation, we used cells (3463T3) isolated from a Slc20a−/− mouse and co-transfection of vectors expressing variant and WT human PiT2 proteins; the 3463T3 cells do not express endogenous PiT2 in the plasma membrane (Jensen et al. submitted manuscript).