HNE and its glutathione conjugates activate signaling through various protein kinase cascades and regulate NF-B and AP-1, lead to develop a number of inflammatory diseases.41 In this study, H&E staining, Oil Red O staining and IHC of 4-HNE of the liver sections revealed an accumulation of lipid droplets and inflammation occurrence in fructose-induced NAFLD mice (Figures 1 and 2). Here, WEE1 is linked to metabolic dysfunction-associated steatotic liver disease.