Rho GTPases are crucial regulators of the cytoskeleton during cell migration and invasion.32 In order to understand how NOX4 is suppressing contractility and the invasive capacity of HCC cells, we analysed expression levels of different Rho GTPases that have been reported to be crucial for sustaining bleb-based migration and actomyosin contractility.6, 7, 11 We found higher messenger RNA (mRNA; Figure 6a) and protein levels (Figure 6b) of RhoA, RhoC and Cdc42 in cells with low NOX4 expression. Here, CDC42 is linked to hepatocellular carcinoma.