HMGB1 and neoplasm: While in MIAPaCa-2 tumor cells no changes in VEGF gene expression were observed, significantly increased gene expression of PDGF was demonstrated after single or combined stimulation with the TLR ligands (ODN: FD = 2.5, p < 0.05; HMGB1: FD = 8.0, p < 0.005; ODN + HMGB1: FD = 2.4, p < 0.05) (Figure 6B).