TLR9 and pancreatic neoplasm: In RT-qPCR, elevated relative gene expression of TLR2 (fold difference, FD = 29.8, p < 0.05), TLR4 (FD = 39.9, p < 0.005), and TLR9 (FD = 10.3, p < 0.005) was observed in pancreatic tumor tissues compared to normal tissues (Figure 1B).