Consistent with prior in vitro investigations of the R141W mutation [8–11], as well as in vitro and in vivo investigations of other TNNT2 DCM mutations, our data suggest that reduced myofilament Ca2+ binding affinity caused by the R141W mutation leads to an adaption of the system by increasing the amplitude of Ca2+ transients. This evidence concerns the gene TNNT2 and familial dilated cardiomyopathy.