Network analysis of this five-gene signature revealed multiple network interactions in CRC, such as between VIP and GNG11, GNB3, GNG12, GNB2, GNG5, GNAS, GNG2, GNB4, GNG4, GNG10, and GNB1; between DACT1 and ARRB1, DVL1, CSNK2B, CSNK2A1, and CSNK2A2; and between S100A2 and TP53 (Fig 2d). This evidence concerns the gene GNG4 and colorectal carcinoma.