ACTA2 and posterior cortical atrophy: As a complementary approach to analyzing human PCa stromal content, we analyzed RNA-sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA) dataset (n=27 normal and 330 primary PCa samples) (Abeshouse et al., 2015), and found a significant decrease in expression of the SM markers ACTA2 and CNN1, as well as the fibroblast marker VIM in PCa compared with normal prostate samples (Fig. 1J).