As a complementary approach to analyzing human PCa stromal content, we analyzed RNA-sequencing (RNA-seq) data from The Cancer Genome Atlas (TCGA) dataset (n=27 normal and 330 primary PCa samples) (Abeshouse et al., 2015), and found a significant decrease in expression of the SM markers ACTA2 and CNN1, as well as the fibroblast marker VIM in PCa compared with normal prostate samples (Fig. 1J). The gene discussed is CNN1; the disease is posterior cortical atrophy.