All four compounds potently inhibited a WNT-dependent reporter in all of the cell lines tested, but did not inhibit a WNT-independent housekeeping EEF1A1 promoter-reporter construct in the negative control RKO colorectal cancer cell line, which expresses low levels of beta-catenin (Figure 1D and Figure 1—source data 1) (Mallinger et al., 2015; Dale et al., 2015). Here, CTNNB1 is linked to colorectal cancer.