However, BIX01294 induced changes could be cell-type specific, as in human colon cancer HCT116 cells the expression of LC3B, ATG9A, ATG4A, but not ATG4B/C, ATG7, BECN1, WIPI1 was increased after BIX01294 treatment34. This evidence concerns the gene ATG4A and malignant colon neoplasm.