BIX01294-induced autophagy was partially blocked by 3-methyladenine and by selective silencing of crucial autophagy genes ATG5, ATG7, ULK1 and BECN1. Similarly, inhibition or knockdown of G9a resulted in increased LC3B, ATG9B and WIPI1 expression, formation of autophagosomes and LC3-II accumulation in cervical cancer HeLa cells and pancreatic cancer SU86.86 cells28. This evidence concerns the gene EHMT2 and familial pancreatic carcinoma.