Co-incubation with BafA1, which prevents fusion of autophagosomes with lysosomes, enhanced BIX01294-induced accumulation of GFP-LC3 punctation (Fig. 1D) and conversion of LC3-I to LC3-II (Supplementary Fig. S1C), which suggests dynamic autophagy in glioma cells upon BIX01294-treatment. This evidence concerns the gene MAP1LC3A and glioma.