Moreover, 2 μM BIX01294 enhanced formation of GFP-LC3 puncta which was suppressed by an autophagy inhibitor (3MA) (Supplementary Fig. S2), suggesting that BIX01294 induces autophagy in established cell lines as well as in the patient-derived glioblastoma stem-like cell cultures. The gene discussed is MAP1LC3A; the disease is glioblastoma.