To evaluate whether up-regulation of the VPMNN system could influence anxiety as measured in the EPM test, and if MS, which had resulted in a hyper-innervation pattern of AVP in the amygdala, could further influence anxiety in adults, we performed this behavior test with the following four groups: AFR basal, MS basal, AFR with 24 h of WD (AFR WD 24 h) and MS with 24 h of WD (MS WD 24 h; Figure 6). The gene discussed is AVP; the disease is Anxiety.