To further assess the potential association between ASPP2 and p65/RelA, NF-κB reporter plasmid was used to examine the NF-κB activity, which was suppressed by overexpression of ASPP2 and enhanced by knockdown of ASPP2 in HCC-LM3 cells (Figure 3e, left and Supplementary Figure 4B). This evidence concerns the gene RELA and hepatocellular carcinoma.