ATG5 and hepatocellular carcinoma: Quantitative real-time PCR showed that the mRNA levels of ATG5, ATG7, and BECN1 were higher in ASPP2-silenced HepG2 and HCC-LM3, which can be rescued by overexpressing ASPP2 in Huh7 and HCC-LM3 (Figure 2a and Supplementary Figure 3).