Interestingly, following the withdrawal of 2-DG, re-stimulation of SCC-1 cells with EGF in fresh medium restored the percentage of CD44+/CD24low/− subpopulation; while during this reversal EMT process, the heterogeneous cancer cells may undergo non-synchronous morphological changes (mesenchymal and epithelial-like morphologies), thus making them shown as two groups on the dot plots of flow cytometric analysis (Figure 3G; the lower right panel). This evidence concerns the gene EGF and cancer.