To further evaluate the role of FOXO1 in HCC cells migration and invasion, we established stable cell lines that were infected with the LV-NC lentivirus (referred to as HCCLM3-Control and SK-HEP-1-Control) or with the LV-FOXO1 lentivirus (referred to as HCCLM3-FOXO1 and SK-HEP-1-FOXO1). This evidence concerns the gene FOXO1 and hepatocellular carcinoma.