IFNB1 and infection: In order to establish whether EAV replication is sensitive to IFN-β treatment in HuH-7 cells, cells were infected with a recombinant green fluorescent protein (GFP)-expressing reporter virus (EAV-GFP [33]; multiplicity of infection [MOI] of 10) and treated with IFN-β from 1 h postinfection (p.i.)onward.