In initial experiments, we assessed the expression of C-type lectin Mincle in WT mice infected with focal pneumonia inducing serotype 19F S. pneumoniae. As shown in Fig 1A, Mincle gene expression was found to peak in lung tissue of mice at 24 h post S. pneumoniae challenge, with a strong decline towards 72 h post-infection. This evidence concerns the gene CLEC4E and susceptibility to pneumonia measurement.