Moreover, SOCS6 was synergistically downregulated in IAV- or IAV/SP-infected MDMs overexpressing miRNA-200a (Fig. 3e), suggesting that both infection and miR-200a-3p negatively regulate the expression of SOCS6. Finally, western blotting confirmed that expression of SOCS-6 sharply reduced following infection, especially after mixed IAV and SP infection (Fig. 3f). Here, SOCS6 is linked to infection.