Since kidney expresses eNOS, iNOS, and nNOS (Prabhakar et al., 2007) and decreased renal NO bioavailability is linked to diabetic renal disease (Erdely et al., 2004; Prabhakar, 2005), the question whether each of these isoenzymes are uncoupled in kidney of obesity mice remains to be investigated. This evidence concerns the gene NOS1 and obesity due to melanocortin 4 receptor deficiency.