In a previous study, we showed that myristylated/activated AKT1 (myr-AKT1) synergizes with the activated form of Notch1 (NICD1) to induce ICC development in mice.22 Thus, we hypothesized that Jag1 overexpression, due to its role as a Notch ligand, might promote ICC development in combination with activated AKT. The gene discussed is NOTCH1; the disease is intrahepatic cholangiocarcinoma.