AKT1 and intrahepatic cholangiocarcinoma: Another important achievement of the present study is the establishment of a novel murine ICC model by hydrodynamic transfection of myr-AKT1 and Jag1. AKT/Jag1-induced ICC tumours exhibited increased cell proliferation and extensive stromal reaction as well as high levels of AKT/mTOR and Notch activation.