AS-IV downregulated the abnormally high expression of TSLP and IL-33 in the initial phase of ACD in vivo and inhibited these two pro-allergic cytokines in HaCaT cells in vitro, which we ascribed to its regulation of key pro-allergic cytokines in the initial stage of ACD, in which AS-IV inhibited ACD. The gene discussed is IL33; the disease is granular corneal dystrophy type II.