NUDT1 and esophageal squamous cell carcinoma: To investigate this, we evaluated MTH1 mRNA expression in the nine ESCC cell lines (TE1, TE2, TE3, TE5, TE8, TE10, TE12, TE13, and TE15) and three human fibroblast cell lines (MRC5, BJ, and WI‐38) by performing qRT‐PCR with two independent primer sets that specifically amplified MTH1 coding region (Fig. 1A).