To evaluate the potential anti-proliferative effect of metformin in cervical carcinoma cells, Cells were treated with 0, 1, 2.5, 5, 10, and 15 mM metformin, or 10 mM metformin with or without 10 ng/mL TGF-β1. The gene discussed is TGFB1; the disease is cervical carcinoma.