The following are the main findings of our study: i) Ado induces a significant increase in cell proliferation and migration, ii) the MDA-MB 231 cells express the four types of Ado receptors, being the A2B type the most abundantly expressed, iii) the Ado-induced increase in proliferation and migration depends on sustained activation of the A2B receptor, which activates the intracellular adenylate cyclase/cAMP-dependent protein kinase (PKA) signaling pathway, and iv) Ado also increases the functional expression of NaV1.5 channels, a potential biomarker in breast cancer. Here, ADCY1 is linked to breast carcinoma.