In Figure 7A, knockdown of YB-1 suppressed Wnt signaling target genes, Axin1 and Axin2. The protein level of β-catenin was also downregulated in both YB-1 KD hepatoma cells and YB-1 KD sphere cells (Figure 7B and Supplementary Figure S2B). This evidence concerns the gene AXIN2 and hepatocellular carcinoma.