Here, we performed the ChIP assay with the antibodies against acetyl-histone 3 and 4 targeting the promoters of p21, p27, Cyclin D1, c-myc and Nanog. It was found that SAHA promoted acetylation of histones 3 and 4 in glioma cells, which were recruited to the promoter of p21, p27, Cyclin D1, c-myc and Nanog for their down-regulated transcription. Here, NANOG is linked to glioma.