To investigate the effect of CYR61 on cancer cell survival under non-adhesive conditions, we maintained NS and CYR61 KD MDA-MB-231 and NS and CYR61 KD SUM159 cells in suspension and monitored induction of cell apoptosis by Annexin V/Near-IR Dead Cell Marker staining and flow cytometry analysis. This evidence concerns the gene ANXA5 and cancer.