To determine the time course of platelet recruitment to the ischemic myocardium, transient myocardial ischemia of 60 min was induced and mice were injected intravenously with a single dose of the scFvanti-GPIIb/IIIa-64CuMeCOSar radiotracer 5 min, 2 or 4 h post cardiac ischemia (pCI) (Fig. 1a,b). Here, ITGA2B is linked to myocardial ischemia.