Since the G2/M transition depends on the formation of the metaphase promotion factor (MPF), CDK1-Cyclin B1 complex, and since Cyclin B1 decreased in HCC, we analysed the changes in protein stability caused by co-treatment of Huh7 cells with heat shock and 17-DMAG (Fig. 6a). This evidence concerns the gene CDK1 and hepatocellular carcinoma.