MOG and demyelinating disease: Firstly, we tagged surface D2R using anti-HA antibody on live WT D2R-transfected HEK293 cells, and then incubated the cells with 8 μg of protein G-purified IgG from controls (CTL), other neurological disorders (OND), demyelinating diseases (DEM) (positive for anti-MOG antibody [14]), MPD anti-D2R antibody-positive sera with similar ΔMFIs, or culture medium alone for 30 min or 2 h at room temperature, or 30 min at 37 °C.