At 6 h after B. melitensis 16M infection, NLRP3 and AIM2 in the O-A and OA-IA cells were significantly higher than in the control cells (P < 0.01), indicating that at 6 h B. melitensis 16M infection activated NLRP3 and the AIM2 inflammasome. The gene discussed is AIM2; the disease is infection.