The gene encoding miR-148a-3p is regulated by epigenetic changes, and miR-148a-3p has been reported to interact with DNMT1.37, 38, 39 In a previous study, DNMT1 could hypermethylate the miR-148a gene promoters in breast cancer tissues, and DNMT1 overexpression resulted in miR-148a hypermethylation.27 In our study, we found that miR-148a-3p surrounding CpG islands were hypermethylated in bladder cancer and that treatment with 5-aza-CdR induced miR-148a-3p overexpression in bladder cancer. The gene discussed is DNMT1; the disease is breast cancer.