CASP2 and acute myeloid leukemia: These results indicate that Casp2−/− BM cells are more susceptible to aneuploidy in vivo with ageing and also may explain the association of reduced caspase-2 expression with poor survival in AML, as aneuploidy is a negative prognostic indicator.32, 33 As ageing is associated with accumulation of DNA damage in HSC,24, 34 we assessed this and observed significantly enhanced basal γH2AX foci together with an increased number of γH2AX-positive cells in aged Casp2−/− BM compared with aged WT cells (Figure 5d and e).