Myoblasts and myotubes in MDC1A and LGMD2D cultures showed FUS staining patterns that were similar to those of healthy controls; and, in FSHD cultures, the large majority of myotube nuclei that did not immunostain for DUX4-FL also had the same pattern of FUS staining as healthy controls (not shown). This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.