To verify that ICON-mediated ADCC is also effective in killing CD24-CD44+ CSCs isolated from the CD24 and CD44-based CSC isolation protocol, we compared the effect of ICON-mediated ADCC assay in killing CD24-CD44+ and CD133+ CSCs isolated from the TNBC MDA-MB-231 line and breast cancer CSCs isolated from patients. This evidence concerns the gene CD24 and breast cancer.