The most promising EVs biomarkers found in the protein combination models, such as ADSV and TGM4 (for the benign vs. PCa comparison) and CD63, GLPK5 and SPHM (for the low-grade vs. high-grade comparison) were further evaluated in patient tissues from radical prostatectomies using tissue microarrays (TMAs). The gene discussed is TGM4; the disease is posterior cortical atrophy.