In agreement with the expression in urine EVs, IHC on prostate TMA-tissues revealed that both PCa diagnostic biomarkers, ADSV and TGM4, were also altered in terms of protein detection (ADSV p < 0.001; TGM4 p < 0.001) when comparing 98 benign prostatic tissues versus 136 PCa tissues (Figure 4A–4B). This evidence concerns the gene TGM4 and posterior cortical atrophy.