GATA6 and malignant colon neoplasm: We thus transfected HCT116 and HT29 colon cancer cells, which express endogenous GATA6 (Supplementary Figure S2B), with the mature miR-196b (miR-196b-5p, Ambion), with a control, unrelated miRNA (Ambion), or with the control, opposite strand miR-196b-3p in HCT116 cells.