IDH1 and central nervous system cancer: As a first approach, we used conventional PCR to amplify IDH1 gDNA sequences, however, IDH1G395A was observed in none of the low-grade gliomas, and only in 4 of the high-grade glioma samples, including HGUGM008, for which the presence of IDH1R132H was detected on a surgical solid sample by IHC (Table 1).