To extend our experiments, we further analyzed NRN1 mRNA expression level from in situ microdissected tissue from melanoma patients (5 primary, 6 lymph node- and 9 visceral metastases) and compared the expression status with NHEMs and microdissected epidermis from normal skin (altogether 7 donors) (Figure 6a). This evidence concerns the gene NRN1 and melanoma.