These results emphasize selectivity differences in how invadopodia are initiated, and the importance of the tumor microenvironment in determining the different invadopodium functions that result from differences in how invadopodia are initiated; chemotaxis during invasive migration involving invadopodium initiation in response to growth factors44, fibronectin directed invasion involving invadopodium initiation by integrin beta-143, and transendothelial migration as described here involving invadopodium initiation via Notch signaling in response to heterotypic cancer cell-macrophage contact. This evidence concerns the gene FN1 and cancer.