Molecular profiling of CTCs in advanced NSCLC was approached by subtracting the background of unspecific immunoisolation from healthy donors (n = 4) to the global gene expression profile of EpCAM-based immunoisolated CTCs from patients (n = 10; Supplementary Table S2), as described by Barbazan et al. 11. This evidence concerns the gene EPCAM and non-small cell lung carcinoma.