The ability of 12 to inhibit tumor cells proliferation was also tested in mouse ASZ001 BCC cells, previously characterized as a specific Hh-dependent tumor cell line harboring Ptch1 deletion.6412 showed significant efficacy to impair ASZ001 BCC cell growth in vitro compared with Cyclopamine, Vismodegib and LDE-225 (Figures 6a and b), in agreement with the decrease of Gli1 mRNA levels observed after drug treatment (Figure 6c). This evidence concerns the gene PTCH1 and skin basal cell carcinoma.