The ability of 12 to inhibit tumor cells proliferation was also tested in mouse ASZ001 BCC cells, previously characterized as a specific Hh-dependent tumor cell line harboring Ptch1 deletion.6412 showed significant efficacy to impair ASZ001 BCC cell growth in vitro compared with Cyclopamine, Vismodegib and LDE-225 (Figures 6a and b), in agreement with the decrease of Gli1 mRNA levels observed after drug treatment (Figure 6c). This evidence concerns the gene PTCH1 and neoplasm.