ATM and infection: In contrast, addition of ATc at the time of infection restored intracellular growth of the VirB conditional strain to wild-type levels, demonstrating full complementation of the virB11 deletion by the ATc-inducible copy of virB11. Under these experimental conditions, VirB11 was detected by Western blotting as early as 1 h postinfection (pi) and throughout a 48 h time course during which ATc treatment was maintained (Fig. 1B, right panels), increasing with bacterial replication past 12 h pi.