Compared to TEM1 fusion to a B. abortus protein (encoded by the BAB2_0654 locus) that is not translocated upon infection (20), at 16 h pi, BspD and BspI showed significant and comparable levels of translocation from strain 2308ΔvirB11::virB11i bacteria treated with ATc either for the whole infection time (ATc16h) or for a 4 h preinduction (ATc−4h) (Fig. 4E). This evidence concerns the gene SPP1 and infection.