2014). The most prevalent variant, with a carrier frequency of 1.6% in cases, identified by Carnes et al. (2014) (rs146737847:G>A) was found in 1.6% of our POAG cases, but also in 1.9% of local screened controls and 1.6% of AOGC controls with an overall OR of 0.98. The other three rare variants found in that study at a carrier frequency of 0.4% were not detected in our cases or controls. In summary, our study found no enrichment of rare variants in SIX6 in our POAG cases versus controls. This evidence concerns the gene SIX6 and open-angle glaucoma.