These results suggest that DC may play an important role in the formation of visceral hypersensitivity in IBS by the activation of spleen CD4+/CD8+ to produce excessive IL-4 and IL-9 under local or systemic stress; knockdown of PDIA3 expression reduces the number and activity of intestinal DCs in visceral hypersensitivity rats, inhibits the abnormal increase of MC in serum and colon and the activity of abnormally secreted cytokines. This evidence concerns the gene CD4 and irritable bowel syndrome.