In our study, knockdown and overexpression of Prdx2 in the colon cancer cell lines SW620, HT29, and HCT116 caused lower and higher CD133+ populations, respectively, as well as concomitant decreased and increased transcription and translation levels of CD44, CD133, Lgr5 and Nanog expression, as well as sphere formation. This evidence concerns the gene CD44 and colonic neoplasm.